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Smedja Bäcklund, Anna
Publications (9 of 9) Show all publications
Nilsson, T., Rova, M. & Smedja Bäcklund, A. (2013). Microbial metabolism of oxochlorates: A bioenergetic perspective. Biochimica et Biophysica Acta - Bioenergetics, 1827(2), 189-197
Open this publication in new window or tab >>Microbial metabolism of oxochlorates: A bioenergetic perspective
2013 (English)In: Biochimica et Biophysica Acta - Bioenergetics, ISSN 0005-2728, E-ISSN 1879-2650, Vol. 1827, no 2, p. 189-197Article, review/survey (Refereed) Published
Abstract [en]

The microbial metabolism of oxochlorates is part of the biogeochemical cycle of chlorine. Organisms capable of growth using perchlorate or chlorate as respiratory electron acceptors are also interesting for applications in biotreatment of oxochlorate-containing effluents or bioremediation of contaminated areas. In this review, we discuss the reactions of oxochlorate respiration, the corresponding enzymes, and the relation to respiratory electron transport that can contribute to a proton gradient across the cell membrane. Enzymes specific for oxochlorate respiration are oxochlorate reductases and chlorite dismutase. The former belong to DMSO reductase family of molybdenum-containing enzymes. The heme protein chlorite dismutase, which decomposes chlorite into chloride and molecular oxygen, is only distantly related to other proteins with known functions. Pathways for electron transport may be different in perchlorate and chlorate reducers, but appear in both cases to be similar to pathways found in other respiratory systems.

Place, publisher, year, edition, pages
Elsevier, 2013
Keywords
Perchlorate reductase, Chlorate reductase, Chlorite dismutase, Respiration, Cytochrome, Electron transport
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-14068 (URN)10.1016/j.bbabio.2012.06.010 (DOI)000314440200010 ()22735192 (PubMedID)
Available from: 2012-06-29 Created: 2012-06-29 Last updated: 2019-12-04Bibliographically approved
Smedja Bäcklund, A. (2011). c Cytochromes as Electron Carriers in Microbial Chlorate Respiration. (Doctoral dissertation). Karlstad: Karlstad University
Open this publication in new window or tab >>c Cytochromes as Electron Carriers in Microbial Chlorate Respiration
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Microbial respiration of oxochlorates is important for the biotreatment of effluents from industries where oxochlorates are produced or handled. Several bacterial species are capable to use perchlorate and/or chlorate as an alternative electron acceptor in absence of oxygen. The present study deals with the electron transport from the membrane-bound components to the periplasmic chlorate reductase, in the gram-negative bacterium Ideonella dechloratans. Both chlorate reductase and the terminal oxidase of I. dechloratans were found to utilize soluble c cytochromes as electron donors. For further investigation, two major heme-containing components were purified and characterized. The most abundant was a 9 kDa c-type cytochrome (class I), denoted cytochrome c-Id1. This protein was shown to serve as electron donor for both chlorate reductase, and for a terminal oxidase. The other major component was a 55 kDa homotetrameric cytochrome c', (class II). A function for this cytochrome could not be demonstrated but it does not appear to serve as electron donor to chlorate reductase. A gene predicted to encode a soluble c cytochrome was found in close proximity to the gene cluster for chlorate reduction. The predicted sequence did not match any of the cytochromes discussed above. The gene was cloned and expressed heterologously, and the resulting protein was investigated as a candidate electron donor for chlorate reductase. Electron transfer from this protein could not be demonstrated, suggesting that the gene product does not serve as immediate electron donor for chlorate reductase.

Place, publisher, year, edition, pages
Karlstad: Karlstad University, 2011. p. 48
Series
Karlstad University Studies, ISSN 1403-8099 ; 2011:40
Keywords
c cytochrome, chlorate reduction, electron transport
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-8038 (URN)978-91-7063-375-1 (ISBN)
Public defence
2011-09-30, Andersalen, 11D 121, Karlstads universitet, Karlstad, 10:15 (Swedish)
Opponent
Supervisors
Available from: 2011-09-06 Created: 2011-08-11 Last updated: 2011-11-14Bibliographically approved
Smedja Bäcklund, A. & Nilsson, T. (2011). Purification and characterization of a soluble cytochrome c capable of delivering electrons to chlorate reductase in Ideonella dechloratans. FEMS Microbiology Letters, 321, 115-120
Open this publication in new window or tab >>Purification and characterization of a soluble cytochrome c capable of delivering electrons to chlorate reductase in Ideonella dechloratans
2011 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 321, p. 115-120Article in journal (Refereed) Published
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-8163 (URN)10.1111/j.1574-6968.2011.02321.x (DOI)000292741300005 ()
Available from: 2011-09-06 Created: 2011-09-06 Last updated: 2017-12-08Bibliographically approved
Bohlin, J., Smedja Bäcklund, A., Gustavsson, N., Wahlberg, S. & Nilsson, T. (2010). Characterization of a cytochrome c gene located at the gene cluster for chlorate respiration in Ideonella dechloratans. Microbiology Research, 165, 450-457
Open this publication in new window or tab >>Characterization of a cytochrome c gene located at the gene cluster for chlorate respiration in Ideonella dechloratans
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2010 (English)In: Microbiology Research, ISSN 0944-5013, E-ISSN 1618-0623, Vol. 165, p. 450-457Article in journal (Refereed) Published
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-8162 (URN)10.1016/j.micres.2009.10.003 (DOI)000282633000002 ()20015627 (PubMedID)
Available from: 2011-09-06 Created: 2011-09-06 Last updated: 2018-01-23Bibliographically approved
Smedja Bäcklund, A. (2009). Electron transport in microbial chlorate respiration. (Licentiate dissertation). Karlstad: Karlstad University
Open this publication in new window or tab >>Electron transport in microbial chlorate respiration
2009 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Several bacterial species are capable to use perchlorate and/or chlorate as an alternative electron acceptor in absence of oxygen. Microbial respiration of oxochlorates is important for biotreatment of effluent from industries where oxochlorates are produced or handled. One of these species, the Gram-negative Ideonella dechloratans, is able to reduce chlorate but not perchlorate. Two soluble enzymes, chlorate reductase and chlorite dismutase, participate in the conversion of chlorate into chloride and molecular oxygen. The present study deals with the electron transport from the membrane-bound components to the periplasmic chlorate reductase. Soluble c cytochromes were investigated for their ability to serve as electron donors to chlorate reductase. The results show that a 6 kDa c cytochrome serves as electron donor for chlorate reductase. This cytochrome also serves as electron donor for a terminal oxidase in the reduction of oxygen that is produced in the course of chlorate respiration. A gene encoding a soluble c cytochrome was found in close proximity to the gene cluster for chlorate reduction. This gene was cloned and expressed heterologously, and the resulting protein was investigated as a candidate electron donor for chlorate reductase. Electron transfer from this protein could not be demonstrated, suggesting that the gene product does not serve as immediate electron donor for chlorate reductase.

 

Place, publisher, year, edition, pages
Karlstad: Karlstad University, 2009. p. 24
Series
Karlstad University Studies, ISSN 1403-8099 ; 2009:18
Keywords
c cytochromes, chlorate reduction, electron transport, c cytokromer, kloratreduktion, elektrontransport
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-3777 (URN)978-91-7063-241-9 (ISBN)
Presentation
2009-04-17, Ericssonsalen, 9C 204, Karlstads universitet, Karlstad, 13:15 (English)
Opponent
Supervisors
Available from: 2009-03-20 Created: 2009-03-09 Last updated: 2011-11-14Bibliographically approved
Smedja Bäcklund, A., Bohlin, J., Gustavsson, N. & Nilsson, T. (2009). Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans. Applied and Environmental Microbiology, 75, 2439-2445
Open this publication in new window or tab >>Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans
2009 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 75, p. 2439-2445Article in journal (Refereed) Published
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-2804 (URN)10.1128/AEM.01325-08 (DOI)
Note

Artikelns titel i Anna Smedja Bäcklunds licentiatuppsats (2009): "Periplasmic c cytochromes coupled to chlorate reduction in Ideonella dechloratans" Artikelns titel i Jan Bohlins doktorsavhandling (2008): "Periplasmic c cytochromes coupled to chlorate reduction in Ideonella dechloratans"

Available from: 2008-10-08 Created: 2008-10-08 Last updated: 2018-01-23Bibliographically approved
Bohlin, J., Smedja Bäcklund, A., Gustavsson, N., Wahlberg, S. & Nilsson, T.Characterization of a candidate cytochome c gene associated with the gene cluster for chlorate respiration in Ideonella dechloratans.
Open this publication in new window or tab >>Characterization of a candidate cytochome c gene associated with the gene cluster for chlorate respiration in Ideonella dechloratans
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(English)Manuscript (Other (popular science, discussion, etc.))
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kau:diva-3828 (URN)
Available from: 2009-03-20 Created: 2009-03-20 Last updated: 2018-01-23Bibliographically approved
Bohlin, J., Smedja Bäcklund, A., Gustavsson, N., Sara, W. & Nilsson, T.Expression, refolding and reconstitution of a c-type cytochrome of Ideonella dechloratans.
Open this publication in new window or tab >>Expression, refolding and reconstitution of a c-type cytochrome of Ideonella dechloratans
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(English)Manuscript (Other academic)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-2800 (URN)
Available from: 2008-10-07 Created: 2008-10-07 Last updated: 2018-01-23Bibliographically approved
Smedja Bäcklund, A. & Nilsson, T.Purification and characterization of a cytochrome c' from the chlorate respiring bacterium Ideonella dechloratans.
Open this publication in new window or tab >>Purification and characterization of a cytochrome c' from the chlorate respiring bacterium Ideonella dechloratans
(English)Manuscript (preprint) (Other academic)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-8164 (URN)
Available from: 2011-09-06 Created: 2011-09-06 Last updated: 2011-11-02Bibliographically approved
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